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Cell viability of <t>L929</t> murine fibroblasts after 48 h of exposure to the leaf essential oils of Psidium guajava L. obtained under different pre‐processing conditions, determined by the MTT assay. Panels A–D correspond to EOFH‐S, EOFH‐M, EOFZ‐S and EOFZ‐M, respectively (concentrations in µg/mL); the horizontal line marks 70% viability. Data are expressed as mean ± SD; differences versus the negative control were assessed by one‐way ANOVA followed by Bonferroni's post hoc test ( n = 3, performed in triplicate; GraphPad Prism 5.0); * p < 0.05 and ** p < 0.01 versus the negative control. Essential oils obtained from fresh leaves fragmented with scissors (EOFH‐S) or ground in a mixer (EOFH‐M). Essential oils obtained from previously frozen leaves fragmented with scissors (EOFZ‐S) or ground in a mixer (EOFZ‐M).
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Cell viability of <t>L929</t> murine fibroblasts after 48 h of exposure to the leaf essential oils of Psidium guajava L. obtained under different pre‐processing conditions, determined by the MTT assay. Panels A–D correspond to EOFH‐S, EOFH‐M, EOFZ‐S and EOFZ‐M, respectively (concentrations in µg/mL); the horizontal line marks 70% viability. Data are expressed as mean ± SD; differences versus the negative control were assessed by one‐way ANOVA followed by Bonferroni's post hoc test ( n = 3, performed in triplicate; GraphPad Prism 5.0); * p < 0.05 and ** p < 0.01 versus the negative control. Essential oils obtained from fresh leaves fragmented with scissors (EOFH‐S) or ground in a mixer (EOFH‐M). Essential oils obtained from previously frozen leaves fragmented with scissors (EOFZ‐S) or ground in a mixer (EOFZ‐M).
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Cell viability of <t>L929</t> murine fibroblasts after 48 h of exposure to the leaf essential oils of Psidium guajava L. obtained under different pre‐processing conditions, determined by the MTT assay. Panels A–D correspond to EOFH‐S, EOFH‐M, EOFZ‐S and EOFZ‐M, respectively (concentrations in µg/mL); the horizontal line marks 70% viability. Data are expressed as mean ± SD; differences versus the negative control were assessed by one‐way ANOVA followed by Bonferroni's post hoc test ( n = 3, performed in triplicate; GraphPad Prism 5.0); * p < 0.05 and ** p < 0.01 versus the negative control. Essential oils obtained from fresh leaves fragmented with scissors (EOFH‐S) or ground in a mixer (EOFH‐M). Essential oils obtained from previously frozen leaves fragmented with scissors (EOFZ‐S) or ground in a mixer (EOFZ‐M).
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Cell viability of <t>L929</t> murine fibroblasts after 48 h of exposure to the leaf essential oils of Psidium guajava L. obtained under different pre‐processing conditions, determined by the MTT assay. Panels A–D correspond to EOFH‐S, EOFH‐M, EOFZ‐S and EOFZ‐M, respectively (concentrations in µg/mL); the horizontal line marks 70% viability. Data are expressed as mean ± SD; differences versus the negative control were assessed by one‐way ANOVA followed by Bonferroni's post hoc test ( n = 3, performed in triplicate; GraphPad Prism 5.0); * p < 0.05 and ** p < 0.01 versus the negative control. Essential oils obtained from fresh leaves fragmented with scissors (EOFH‐S) or ground in a mixer (EOFH‐M). Essential oils obtained from previously frozen leaves fragmented with scissors (EOFZ‐S) or ground in a mixer (EOFZ‐M).
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Cell viability of <t>L929</t> murine fibroblasts after 48 h of exposure to the leaf essential oils of Psidium guajava L. obtained under different pre‐processing conditions, determined by the MTT assay. Panels A–D correspond to EOFH‐S, EOFH‐M, EOFZ‐S and EOFZ‐M, respectively (concentrations in µg/mL); the horizontal line marks 70% viability. Data are expressed as mean ± SD; differences versus the negative control were assessed by one‐way ANOVA followed by Bonferroni's post hoc test ( n = 3, performed in triplicate; GraphPad Prism 5.0); * p < 0.05 and ** p < 0.01 versus the negative control. Essential oils obtained from fresh leaves fragmented with scissors (EOFH‐S) or ground in a mixer (EOFH‐M). Essential oils obtained from previously frozen leaves fragmented with scissors (EOFZ‐S) or ground in a mixer (EOFZ‐M).
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Cell viability of <t>L929</t> murine fibroblasts after 48 h of exposure to the leaf essential oils of Psidium guajava L. obtained under different pre‐processing conditions, determined by the MTT assay. Panels A–D correspond to EOFH‐S, EOFH‐M, EOFZ‐S and EOFZ‐M, respectively (concentrations in µg/mL); the horizontal line marks 70% viability. Data are expressed as mean ± SD; differences versus the negative control were assessed by one‐way ANOVA followed by Bonferroni's post hoc test ( n = 3, performed in triplicate; GraphPad Prism 5.0); * p < 0.05 and ** p < 0.01 versus the negative control. Essential oils obtained from fresh leaves fragmented with scissors (EOFH‐S) or ground in a mixer (EOFH‐M). Essential oils obtained from previously frozen leaves fragmented with scissors (EOFZ‐S) or ground in a mixer (EOFZ‐M).
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Cell viability of L929 murine fibroblasts after 48 h of exposure to the leaf essential oils of Psidium guajava L. obtained under different pre‐processing conditions, determined by the MTT assay. Panels A–D correspond to EOFH‐S, EOFH‐M, EOFZ‐S and EOFZ‐M, respectively (concentrations in µg/mL); the horizontal line marks 70% viability. Data are expressed as mean ± SD; differences versus the negative control were assessed by one‐way ANOVA followed by Bonferroni's post hoc test ( n = 3, performed in triplicate; GraphPad Prism 5.0); * p < 0.05 and ** p < 0.01 versus the negative control. Essential oils obtained from fresh leaves fragmented with scissors (EOFH‐S) or ground in a mixer (EOFH‐M). Essential oils obtained from previously frozen leaves fragmented with scissors (EOFZ‐S) or ground in a mixer (EOFZ‐M).

Journal: Chemistry & Biodiversity

Article Title: Impact of Plant Pre‐Processing on the Chemical Profile of Psidium guajava L. (Myrtaceae) Essential Oil and Evaluation of Its Bioactive Potential

doi: 10.1002/cbdv.71843

Figure Lengend Snippet: Cell viability of L929 murine fibroblasts after 48 h of exposure to the leaf essential oils of Psidium guajava L. obtained under different pre‐processing conditions, determined by the MTT assay. Panels A–D correspond to EOFH‐S, EOFH‐M, EOFZ‐S and EOFZ‐M, respectively (concentrations in µg/mL); the horizontal line marks 70% viability. Data are expressed as mean ± SD; differences versus the negative control were assessed by one‐way ANOVA followed by Bonferroni's post hoc test ( n = 3, performed in triplicate; GraphPad Prism 5.0); * p < 0.05 and ** p < 0.01 versus the negative control. Essential oils obtained from fresh leaves fragmented with scissors (EOFH‐S) or ground in a mixer (EOFH‐M). Essential oils obtained from previously frozen leaves fragmented with scissors (EOFZ‐S) or ground in a mixer (EOFZ‐M).

Article Snippet: The L929 fibroblast cell line (NCTC clone 929; ATCC CCL‐1; RRID:CVCL_0462; American Type Culture Collection, Manassas, VA, USA) was cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% FBS and the same antibiotic solution, at a seeding density of 5 × 10 3 cells per well.

Techniques: MTT Assay, Negative Control